Part:BBa_K2588011

Acetyl-CoA biotin carboxyl carrier protein

Usage and Biology

The Acetyl-CoA biotin carboxyl carrier protein is part of the acetyl-CoA carboxylase ACC which is the major regulator for fatty acid biosynthesis in E. coli. It is encoded as a single mRNA with the downstream accC, which is encoding for the biotin carboxylase of the complex.¹ The acetyl-CoA biotin carboxyl carrier protein binds with biotin and transfers carboxylated biotin to the acetyl-CoA carboxyltransferase.²

The acetyl-CoA biotin carboxyl carrier protein was used in our myristic acid synthesis module, BBa_K2588022.

References

1. Li, S. J. & Cronan, J. E. The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267, 855–63 (1992).
2. Broussard, T. C., Price, A. E., Laborde, S. M. & Waldrop, G. L. Complex formation and regulation of escherichia coli acetyl-CoA carboxylase. Biochemistry (2013). doi:10.1021/bi4000707

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 432
  
• 1000
  COMPATIBLE WITH RFC[1000]